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Indian Journal of Small Ruminants (The)
Year : 2019, Volume : 25, Issue : 1
First page : ( 41) Last page : ( 44)
Print ISSN : 0971-9857. Online ISSN : 0973-9718.
Article DOI : 10.5958/0973-9718.2019.00002.3

In Vitro Fertilization of sheep oocytes with patanwadi ram semen

Jyotsana Basanti*, Krishnappa B., Bahire S., Meena A.S., Kumar D., Naqvi S.M.K., Kumar S.

ICAR-Central Sheep and Wool Research Institute, Avikanagar-304 501, Rajasthan

*E-mail address: bjyotsana@gmail.com

Online published on 4 April, 2019.

Abstract

A study was conducted to develop a culture system and to investigate the effect of modified Charles Rosenkarns 2 (mCR2) and modified Synthetic Oviductal Fluid (mSOF) media on in vitro production of ovine embryos. A total of 570 oocytes were obtained from 297 slaughter-derived ovaries with an average retrieval rate of 1.92 oocytes/ovary by puncturing method. For in vitro fertilization (IVF), oocytes were matured in TCM-199 media supplemented with growth factors for 27 h. The oocytes were incubated with processed and CASA-tested Patanwadi ram semen for 1618 h in Tyrode's albumin lactate pyruvate (TALP) medium for IVF, thereafter it was cultured up to 8 days post insemination in mSOF culture medium for production of blastocysts. Cleavage and morulae formation rate were 12.74±4.43% and 3.10±6.70% in mSOF and 7.26±1.03%, 2.21±0.38% in mCR2 media, respectively. However, these results were statistically nonsignificant (P<0.05). The study indicated that mSOF media was found better than mCR2 in IVF of sheep oocytes.

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Keywords

Fertilization, In vitro embryo production, Oviductal fluid, Sheep.

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