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Indian Journal of Small Ruminants (The)
Year : 2017, Volume : 23, Issue : 1
First page : ( 81) Last page : ( 83)
Print ISSN : 0971-9857. Online ISSN : 0973-9718.
Article DOI : 10.5958/0973-9718.2017.00010.1

PCR-SSCP analysis of exon 2 of Myostatin (MSTN) gene in Marwari goats

Khichar Jai Prakash*, Gahlot Gyan Chand,  Kiran1, Agrawal Vijay Kumar, Gahlot Kritika2

Department of Animal Genetics and Breeding, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan

1Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan

2Department of Veterinary Microbiology, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan

*E-mail address: jai4me007@gmail.com

Online published on 9 March, 2017.

Summary

A study was conducted with the objective to detect genetic polymorphism in exon 2 of myostatin (MSTN) gene in Marwari goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. Genomic DNA was isolated from blood of 120 randomly selected Marwari goats maintained in field units of All India Coordinated Research Project on Goat Improvement. The exon 2 of MSTN gene was amplified using specific primer designed from caprine MSTN gene sequence (Gene bank accession no. DQ167575). SSCP was carried out on 8% non-denaturing polyacrylamide gel to resolve the different genotypic pattern. Exon 2 of MSTN gene revealed two types of conformation patterns. The SSCP pattern that showed only two bands was designated as AA genotype, whereas the other pattern with an extra band was considered as AB genotype. The frequencies of AA and AB genotypes were 0.85 and 0.15, respectively. The dimorphic pattern observed for exon 2 of MSTN gene could be utilized to identify and characterize Marwari goats.

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Keywords

Marwari goat, Myostatin gene, Polymerase chain reaction, Single strand conformation polymorphism.

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