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Indian Journal of Small Ruminants (The)
Year : 2014, Volume : 20, Issue : 1
First page : ( 54) Last page : ( 58)
Print ISSN : 0971-9857. Online ISSN : 0973-9718.

Incidence of Mycobacterium avium subspecies Paratuberculosis in Patanwadi sheep

Shroff S., Chandel B.S., Dadawala A.I., Gupta S.1, Chaubey K.K.1, Singh S.V.1,*

Department of Microbiology, College of Veterinary Science and Animal Husbandry Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar- 385 506, Gujarat

1Microbiology Laboratory, Animal Health Division Central Institute for Research on Goats, Makhdoom- 281 122, Uttar Pradesh

*E-mail address: shoorvir_singh@rediffmail.com

Online published on 5 March, 2014.

Abstract

Incidence of Mycobacterium avium subspecies paratuberculosis (MAP) infection was estimated in Patanwadi sheep at Livestock Research Station, Sardarkrushinagar (Gujarat), using multiple tests. More than 50.0% of Patanwadi sheep had clinical signs (reduced appetite, diarrhoea, weight loss, emaciation, rough wool coat and leathery skin) of paratuberculosis. Of 450 sheep, 133 were screened to estimate bio-load of MAP, wherein 50.3% were positive by indigenous ELISA. Faeces of 50 sheep positive in i-ELISA were screened by microscopy and 13 (26.0%) were positive for acid fast bacilli (AFB) indistinguishable from MAP. Of 13 AFB positive faecal samples in direct microscopy, 3 (23.0%) were also positive on IS900 faecal PCR. Bio-load of MAP (bactremia) was estimated in 50 i-ELISA positive sheep by IS900 blood PCR and 8 (16.0%) were found positive. This is the first study on incidence of MAP in Patanwadi sheep.

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Keywords

Incidence, Mycobacterium avium subspecies paratuberculosis, Patanwadi sheep.

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