Studies on antimicrobial resistance in Escherichia coli isolated from broilers Arpit1, Chandra M.1*, Kaur G.1, Narang D.1, Arora A.K.1 1Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004 *Corresponding Author Email: drmuditchandra@rediffmail.com
Abstract Arpit, Chandra, M., Kaur, G., Narang, D. and Arora, A.K. 2020. Studies on antimicrobial resistance in Escherichia coli isolated from broilers. Indian Journal of Poultry Science, 55(3): 239-242.E. coli a member of Enterobacteriaceae family is a gram-negative, bacilli. In the present study, a total of 50 faecal samples from poultry were processed for the isolation of E. coli. Out of 50 faecal samples tested for isolation of E. coli using selective media, a total of 35 faecal samples had E. coli. All of these isolates were identified and confirmed using selective media, appropriate biochemical tests and genus specific PCR. All the E. coli isolates were tested against 20 different antibiotics to understand their sensitivity and resistance pattern and it was observed that majority of E. coli isolates were found resistant to penicillin, methicillin, ampicillin/ sulbactum and fusidic acid (100%), erythromycin (94.28%), streptomycin (91.4%), furazolidone (82.85%), doxycycline (68.5%), nalidixic acid (65.71%), enrofloxacin (62.8%), tetracycline (60%), chloramphenicol (60%), ciprofloxacin (54.28%), trimethoprim (51.4%), co-trimoxazole (48.57%), neomycin (31.42%) and ceftriaxone (25.71%) Low levels of resistance were against colistin, amikacin, gentamicin (8.5%) and sensitive to gentamicin, colistin (91.5%) ceftriaxone (71.42%), neomycin (68.57%), co-trimoxazole (51.42%), amikacin (45.71%), trimethoprim (45.71%), enrofloxacin (37.14%), doxycycline (31.42%), tetracycline (31.42%), furazolidone (17.14%), naldixic acid (11.42%), chloramphenicol (5.71%), streptomycin (2.85%). Thus, from the study it could be conclude that less usage of antibiotics on farms is required to prevent development and spread of antibiotic resistance bacteria such as E. coli. Top Keywords E. coli, Poultry, Prevalence, Antibiotic Resistance. Top |