Polymorphism analysis of Hsp70 gene in chicken and duck
John Lijo, Panicker Varuna P., George Sisilamma*
Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur - 680 586 (Kerala)
*Corresponding author: E.mail - email@example.com
Online published on 29 April, 2013.
In the present study, the promoter and 3’ untranslated (3’UTR) regions of hsp70 gene was analyzed in different breeds of chicken i.e. Naked neck, Kadaknath, White Leghorn, Rhode Island Red and White Plymouth Rock and duck i.e. Kuttanadu and White Pekin. Both the regions were amplified, cloned and sequenced. Promoter region was found to be conserved in all the breeds and between species. On sequence analysis, point mutations could be identified in the CAAT box of all breeds of chicken and duck except Naked neck and in the HSE motif of White Plymouth Rock. Restriction analysis with 12 restriction enzymes showed a restriction site only for Bgl2 and revealed three alleles in the breeds studied. Analysis of 3’ UTR showed varying levels of sequence polymorphisms. Point mutations were observed in poly adenylation signal sequence and K box and Brd box associated with CAAC sequence. A second variant of polyadenylation signal sequence was observed in Naked neck genotype. Several AT rich motifs were also identified in both the species. Restriction analysis showed a restriction site for Bgl22 and Bam H2 in chicken and ducks respectively, revealing the presence of two alleles.
Hsp70, promoter region, 3’ UTR, Naked neck.