Indian Journal of Poultry Science
Year : 2012, Volume : 47, Issue : 1
First page : ( 90) Last page : ( 92)
Print ISSN : 0019-5529. Online ISSN : 0974-8180.

Studies on absolute requirement of NAD and reduced oxygen tension for growth of field isolates of Avibacterium paragallinarum of poultry origin

Kumar A.*, Rawat M., Verma R.

Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar - 243 122 (U.P.)

*Corresponding author: e-mail: vets_dr_amits@yahoo.co.in

Online published on 11 March, 2013.

Abstract

Now a days there are increasing reports of isolations of NAD-independent Avibacterium paragallinarum, causative agent of much economically important disease of poultry. Infectious Coryza, from different parts of world like South Africa and Mexico. This causes confusion in V-(NAD) factor requirement of the organism which is the most important biochemical property of the organism and is of prime importance while confirming the organism using conventional biochemical methods. Present study was undertaken to investigate the NADdependence and reduced oxygen tension requirement, another very important growth characteristic of the organism, for our field isolates of A. paragallinarum. Field isolates from Barwala area of Panchkula, Haryana and Dera Bassi area of Punjab states were included in the study. Also, the isolates were compared with the confirmed reference strains of the organism procured from elsewhere. Different media supplying and lacking NAD and incubations under different conditions were tried to find out the necessary growth conditions for our field isolates. All the isolates were found to be absolutely requiring the external supplementation of NAD and also all the isolates as well as reference strains failed to grow in the absence of an environment of reduced O₂ tension.

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Keywords

NAD, reduced O₂ tension, Avibacterium paragallinarum.

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