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Indian Journal of Public Health Research & Development
Year : 2018, Volume : 9, Issue : 12
First page : ( 1449) Last page : ( 1454)
Print ISSN : 0976-0245. Online ISSN : 0976-5506.
Article DOI : 10.5958/0976-5506.2018.02057.0

Expression of TLR-4 in Salmonella typhi-Induced Balb/c Mice Treated by Miana Leaves (Coleus scutellaroides (L) Benth)

Syamsuri Fadhilah1,2,*, Hatta Mochammad1, Natzir Rosdiana3, Alam Gemini4, Massi Muhammad Nasrum1, Bahar Burhanuddin5, Rahardjo Sutji Pratiwi6

1Molecular Biology and Immunology Laboratory Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

2Diploma of Cardiovascular Program, Mega Rezky Health Higher School, Makassar, South Sulawesi, Indonesia

3Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

4Phytochemical Laboratory Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia

5Department of Biostatics, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

6Department of Otorhinolaringology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

*Correspondence author: Fadhilah Syamsuri Email: dr_dhilah02@yahoo.co.id

Online published on 2 February, 2019.



Miana is one of the most widely used medicinal plants in Indonesia because its antibacterial activity, but its mechanisms not clear. This study aims to determine mRNA TLR-4 expression in Balb/c Mice induced by Salmonella typhi after miana leaf extract (MLE) treatment.

Material and Method

Mice were divided into four groups, antibiotic (levofoxacin eight mg/kg body weight) as positive control, placebo (Na CMC), MLE and mixed of MLE and antibiotic. Blood of the mice was taken before (H0) and after induction of 103 CFU/mL Salmonella typhi (H1) and seven days after intervention (H7). The mRNA TLR-4 expression was measured by real time PCR. The results obtained were processed using SPSS.


There were signifcant difference in HO-Hl for all groups. TLR-4 expression in Hl-H7 had a different pattern between placebo and positive control, MLE, and the mixed of MLE and antibiotic. In placebo, there was an increase of TLR-4 expression in H1-H7). In the positive control, MLE treatment group and the mixed of MLE and antibiotic, there was a decrease in TLR-4 mRNA expression.


Miana leaves treatment in Balb/c mice induced by Salmonella typhi signifcantly gave the same effect as positive control to expression of mRNA TLR-4.



Toll-like Receptor-4 mRNA, Typhoid Fever, Miana, Real-time PCR, CFU.


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