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Indian Journal of Horticulture
Year : 2004, Volume : 61, Issue : 3
First page : ( 206) Last page : ( 208)
Print ISSN : 0972-8538.

In vitro regeneration in pomegranate cv. Ganesh from mature trees

Murkute A.A.1,*, Patil Shanti2, Singh S.K.3

1Department of Horticulture, College of Agriculture, Nagpur 440 001, Maharashtra

2Department of Genetics and Plant Breeding, College of Agriculture, Nagpur 440 001, Maharashtra

3Division of Fruits and Horticultural Technology, IARI, Pusa, New Delhi

*Corresponding author's present address: Indian Institute of Technology Delhi, New Delhi-110 016; E-mail: ashutoshmurkute@rediffmail.com

Received:  June,  2003; Revised:  January,  2004; Accepted:  August,  2004.

Abstract

An in vitro regeneration studies in pomegranate using shoot tip and nodal segment explants from selected mature pomegranate trees was attempted. The browning of cultures was controlled by regular subsulturing of explants onto media supplemented with same hormonal concentrations. The shoot proliferation was obtained on MS basal medium supplemented with BAP 1.0 mg l−1 + NAA 0.5 mg l−1. The elongated shoots were separated and subcultured for rooting individually on half-strength MS basal medium supplemented with 0.5 mg l−1 either NAA or IAA. The plantlets were transferred to vermicompost + soil mix (1:1) and were hardened in mist chamber with 50% survival.

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Key words

In vitro propagation, mature trees, pomegranate.

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