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Indian Journal of Gerontology
Year : 2021, Volume : 35, Issue : 1
First page : ( 1) Last page : ( 7)
Print ISSN : 0971-4189.

Properties of lipase and lipolytic activity during ageing of female moth, helicoverpa armigera (Hubner)

Gejage R.M., Pawar Aishwarya S.1

Department of Zoology, K.R.P. Kanya Mahavidyalaya, Islampur Tal. Walwa, Dist. Sangli-415409, M. S.

1Department of Zoology, V.Y. Arts and Science College, Pethvadgaon, Tal. Hatkangale, Dist. Kolhapur-416112, M. S.

Online published on 10 September, 2021.

Abstract

Properties of lipase in female moth H. armigera showed optimum pH 8.1, incubation time 20 minutes, temperature 37E C, enzyme concentration 1 per cent, substrate 6 per cent as optimum substrate concentration and Michaelis-Menten constant (Km) value 0.246×10-2 mM. The lipase hydrolysed the triglycerides to diglycerides and fatty acids. The gradual increase in lipolytic activity was noted from 1-day to 5-day and decrease from 5 to 9-day female moth. The maximum lipolytic activity in 5-day old female adults of H. armigera. Arithmetic mean and standard deviation were noted to be 0.3672 and 0.04135 respectively. One way analysis of variance (ANOVA) of lipolytic activity from male and female adults of H. armigera noted to be p<0.003. The role of lipase in female moth of H. armigera has been discussed in present work.

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Keywords

Lipase activity, Female moth, Insect, Ageing, H. armigera.

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