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Indian Journal of Field Veterinarians (The)
Year : 2014, Volume : 9, Issue : 4
First page : ( 46) Last page : ( 53)
Print ISSN : 0973-3175. Online ISSN : 0976-1918.

Development and validation of RP-HPLC method for the estimation of bumetanide in tablet formulation

Sethi Reeta*, Paul Yash**, Goyal Surinder

Lord Shiva College of Pharmacy, Sirsa (Haryana) VidyaSagar Institutes, Sardulgarh (Punjab)

*Corresponding Author: reetasethi05@gmail.com


Online published on 19 June, 2014.


A remarkably simple, selective and precise method to determine bumetanide in tablet dosage form was developed and validated using Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The LC separations was achieved on a bondapak C18 column in the isocratic mode using Glacial acetic acid: Tetrahydrofuran: Water: Methanol (2:5:45:50 v/v/v/v), as a mobile phase at flow rate 1.0 mL/min with UV detection at 254 nm. The retention time for bumetanide was 11.3 min. The method was validated by determining its sensitivity, precision, accuracy as per ICH guidelines. The % recovery was found to be as is simple, rapid, precise and accurate and hence, can be applied for routine quality control analysis of this drug in tablet dosage form.



High performance chromatography, Bumetanide, Validation, Accuracy.


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