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Indian Journal of Field Veterinarians (The)
Year : 2014, Volume : 9, Issue : 4
First page : ( 46) Last page : ( 53)
Print ISSN : 0973-3175. Online ISSN : 0976-1918.

Development and validation of RP-HPLC method for the estimation of bumetanide in tablet formulation

Sethi Reeta*, Paul Yash**, Goyal Surinder

Lord Shiva College of Pharmacy, Sirsa (Haryana) VidyaSagar Institutes, Sardulgarh (Punjab)

*Corresponding Author: reetasethi05@gmail.com

**ypsingla@yahoo.co.in

Online published on 19 June, 2014.

Abstract

A remarkably simple, selective and precise method to determine bumetanide in tablet dosage form was developed and validated using Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The LC separations was achieved on a bondapak C18 column in the isocratic mode using Glacial acetic acid: Tetrahydrofuran: Water: Methanol (2:5:45:50 v/v/v/v), as a mobile phase at flow rate 1.0 mL/min with UV detection at 254 nm. The retention time for bumetanide was 11.3 min. The method was validated by determining its sensitivity, precision, accuracy as per ICH guidelines. The % recovery was found to be as is simple, rapid, precise and accurate and hence, can be applied for routine quality control analysis of this drug in tablet dosage form.

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Keywords

High performance chromatography, Bumetanide, Validation, Accuracy.

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