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Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2015, Volume : 36, Issue : 1
First page : ( 22) Last page : ( 27)
Print ISSN : 0970-9320. Online ISSN : 0974-0147.
Article DOI : 10.5958/0974-0147.2015.00005.7

Analysis of mecA Gene and Antibiotic Resistance in Staphylococcus aureus Isolates from Bovine Mastitis

Kutar K.*, Verma A.K., Sharma B., Kumar Amit1, Yadav S. K.1

Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwavidyalaya, Evum Go-Anusandhan Sansthan (DUVASU), Mathura, India

1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay, Pashu Chikitsa Vigyan Vishwavidyalaya Evum Go-Anusandhan, Sansthan (DUVASU), Mathura, India

*Corresponding Author: E-mail: kutarkiran@gmail.com

Online published on 17 October, 2015.


The objective of the present study was to analyze the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) from cattle and buffaloes with clinical or subclinical mastitis in Uttar Pradesh, India. Out of total 125 mastitic milk sample collected from clinical and subclinical cases of mastitis from cows (109), buffaloes (16), S. aureus was isolated from only 52 samples showing overall incidence of 41.6%. The incidence of S. aureus was higher (56%) in clinical mastitis than in subclinical (20%). A very high percent of isolates were resistant to cotrimoxazole (63.46%), followed by streptomycin (57.69%), gentamicin (55.76%), cephalexine (42.30%), amoxycillin (38.46%) and erythromycin (36.53%). S. aureus isolates were analyzed by PCR for the presence of mecA gene. The presence of mecA gene considered as a molecular marker of methicillin resistance and was detected only in 5 (9.61%) of 52 S. aureus isolates by PCR. The continuous epidemiological studies for antimicrobial resistance patterns of MRSA isolated from animals would be an important measure in detecting emergence and spreading of antibiotic resistance.



Bovine mastitis, Methicillin resistance Staphylococcus aureus, Staphylococcal cassette chromosome mec typing.


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