Detection and isolation of Chicken Infectious Anaemia Virus (CIAV) from field samples of poultry flocks from Gujarat and Andhra Pradesh, India Praveen B.N., Dhama K.*, Kataria J.M., Dash B.B., Singh S.D. Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar - 243 122 (U.P.) *Corresponding author: e.mail - kdhama@rediffmail.com
Abstract Chicken infectious anaemia virus (CIAV), a member of the genus Gyrovirus of the family Circoviridae, is the causative agent of chicken infectious anaemia (CIA), an immunosuppressive disease mainly of young chickens causing considerable economic losses to the poultry industry worldwide. The present paper reports the detection and isolation CIAV from affected poultry flocks of Gujarat and Andhra Pradesh States, India. The disease has been reported in India, but the magnitude of infection in the poultry flocks of the country is not known, warranting the need for ascertaining epidemiological status of the disease in the country. PCR detection of CIAV in 32 field samples, revealed 18 samples to be positive for the virus (16/27 samples positive from Gujarat, and 2/5 positive samples from Andhra Pradesh). CIAV isolate was successfully recovered in MDCC-MSB1 cell cultures from PCR positive field material of Gujarat State. Detection of CIAV in poultry flock of Gujarat and Andhra Pradesh in the present paper adds to the earlier reports regarding to the presence of virus circulating in the poultry population of the country. Due to its high sensitivity, specificity and speed of detection, PCR should be fully explored for studying molecular epizootiology of CIA. These findings, highlights the occurrence of CIA in the country, therefore, focus should be made on CIAV epidemiology to determine the prevalence of the virus in the poultry flocks of the country, and devise suitable prevention and control strategies for this economically important avian pathogen. Top |