Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2006, Volume : 27, Issue : 1
First page : ( 5) Last page : ( 8)
Print ISSN : 0790-9320.

Rapd and masa assay to detect polymorphism for response to infectious bursal disease vaccine in chicken

Raghuvanshi M.¹, Jackeray Richa¹, Sharma Neha¹, Tomar Simmi¹, Dhama K.^1,2, Sharma Deepak^1,*

¹Avian Biotechnology Division, Central Avian Research Institute, Izatnagar - 243 122 (U.P.)

²Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar – 243 122 (U.P.)

*Corresponding author.

Abstract

The genetic polymorphism between extreme responders differing for their immunosuppressiveness against Infectious Bursal Disease (IBD) virus in chicken was detected using Randomly Amplified Polymorphic DNA (RAPD) and Minisatellite/Microsatellite Associated Sequence Amplification (MASA) markers. The population mean antibody titre was 1838.14±27.20, while mean antibody titre in high antibody titre (HAb) line and low antibody titre (LAb) line was 2312.9±22.96 and 1367.70±28.52, respectively. Two of the 16 random primers tested (~12%) could detect polymorphism between the HAb and LAb groups. While primer P11 amplified two polymorphic bands and P₁₄ primer amplified only one polymorphic band. Out of these three polymorphic bands, only one band i.e. P₁₁-1824 was specific to LAb group with a frequency of 1.00. Out of three MASA primers, only GTG₅ was polymorphic between the groups. The GTG₅ amplified two polymorphic loci and one of these two i.e. GTG₅-1830 was specific for HAb group. However, comparatively lower proportion (~12%) of RAPD primers were found to be polymorphic as compared to MASA primers (~33%), but the proportion of polymporphic loci amplified was higher (25%) with RAPD primers as compared to MASA primers (20%).

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