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Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2003, Volume : 24, Issue : 2
First page : ( 133) Last page : ( 135)
Print ISSN : 0970-9320. Online ISSN : 0974-0147.

Comparative efficacy of three serological tests for antigenic characterization of fmd virus type ‘o’ isolates

Sarma Sanjoy1, Sarma D.K.

AICRP on FMD Regional Research Centre, Assam Agricultural University, Khanapara Campus, Guwahati–781 022 (ASSAM)

1Dept. of Veterinary Microbiology

Received:  25  May,  2004.

Abstract

Three serological tests viz. micro complement fixation test (mCFT), soundwich enzyme-linked immunosorbent assay (sELISA) and liquid phase blocking ELISA (LPBE) were used to compare the r value for characterization of 10 isolates of FMD virus type O from field outbreaks with the IVRI vaccine virus strain. In unidirectional testing, the r value of most of the isolates was highest with sELISA and the lowest with the mCFT in unidirectional testing. In the reverse direction testing, the r value of the reference virus strain was almost same with the three tests except the lower r value of the reference strain with two of the isolates antisera in LPBE and with one of the isolate in mCFT.

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