Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2003, Volume : 24, Issue : 2
First page : ( 133) Last page : ( 135)
Print ISSN : 0970-9320. Online ISSN : 0974-0147.

Comparative efficacy of three serological tests for antigenic characterization of fmd virus type ‘o’ isolates

Sarma Sanjoy¹, Sarma D.K.

AICRP on FMD Regional Research Centre, Assam Agricultural University, Khanapara Campus, Guwahati–781 022 (ASSAM)

¹Dept. of Veterinary Microbiology

Received:  25  May,  2004.

Abstract

Three serological tests viz. micro complement fixation test (mCFT), soundwich enzyme-linked immunosorbent assay (sELISA) and liquid phase blocking ELISA (LPBE) were used to compare the r value for characterization of 10 isolates of FMD virus type O from field outbreaks with the IVRI vaccine virus strain. In unidirectional testing, the r value of most of the isolates was highest with sELISA and the lowest with the mCFT in unidirectional testing. In the reverse direction testing, the r value of the reference virus strain was almost same with the three tests except the lower r value of the reference strain with two of the isolates antisera in LPBE and with one of the isolate in mCFT.

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