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Indian Journal of Animal Research
Year : 2022, Volume : 56, Issue : 12
First page : ( 1523) Last page : ( 1529)
Print ISSN : 0367-6722. Online ISSN : 0976-0555.
Article DOI : 10.18805/IJAR.BF-1431

Dexmedetomidine associated lung cancer proliferation and tumor growth is prevented by β-caryophyllene: Role of AMPK activation and PGC-1α/TFAM over expression

Wang Dan1,**, Long Dazhi2,**, Zhou Jiegang1, Dong Ziqiang1, Huang Guiming1,*

1Department of Anesthesiology, Ganzhou People's Hospital, Ganzhou, Jiangxi, 341000, China

2Department of Urology, Ji'an Central Hospital, Ji'an, Jiangxi, 343000, China

*Corresponding Author: Guiming Huang, Department of Anesthesiology, Ganzhou People's Hospital, Ganzhou, Jiangxi, 341000, China, Email: 154093876@qq.com

**These authors contributed equally to this work.

Online Published on 31 December, 2022.

Abstract

Background

Dexmedetomidine has been reported to induce anti-apoptotic effects and metastatic progression in lung cancer. In the current investigation, the effect of p-Caryophyllene on dexmedetomidine induced cell proliferation and apoptosis of lung cancer cells and tumor growth in mice was studied.

Methods

A549 cell line was cultured with either dexmedetomidine alone or together with p-Caryophyllene for 24 h and analysed for cell proliferation with MTT assay. ELISA based kit was used to determine apoptotic DNA fragmentation. Western blotting was used to determine expression levels of target proteins. The induction of experimental lung tumor in rat model was achieved through the injection of A549 tumor cells subcutaneously into the middle left side of the mice after anesthetization with pentobarbital (35 mg/kg) at 2.8x106 cells in 400 |jl of PBS.

Result

We found that p-Caryophyllene exerts the anti-proliferative effects on A549 cells. Furthermore, p-Caryophyllene significantly prevents apoptotic cell death and causes up-regulation of PGC-1a and TFAM compared to dexmedetomidine treated cells. We observed that p-Caryophyllene suppressed tumor development in mice significantly compared to dexmedetomidine treated group without changing body weight.

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Keywords

Apoptosis, P-caryophyllene, Dexmedetomidine, Lung cancer, Tumor growth.

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