Protective effect of Fragarria ananassa and Vaccinium corymbosum fruit extracts against L-arginine induced acute pancreatitis in rats Gadicherla Veena1,2, Challa Siva Reddy3,*, Rao Mandava V. Basaveswara4, Ramakrishna P.5, Kumar K. Pavan6 1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Krishna University, Machilipatnam-521 001, Andhra Pradesh 2Department of Pharmacology, Sri Indu Institute of Pharmacy, Ibrahimpatnam, 501 510, Ranga Reddy District, Andhra Pradesh 3Department of Pharmacology, KVSR Siddhartha Institute of Pharmaceutical Sciences, Vijayawada-520 010, Andhra Pradesh 4Department of Chemistry, Faculty of Pharmaceutical Sciences, Krishna University, Machilipatnam-521 001, Andhra Pradesh 5Faculty of Pharmaceutical Sciences, Krishna University, Machilipatnam-521 001, Andhra Pradesh 6Department of Pharmaceutical Analysis, Shadan College of Pharmacy, Hyderabad-500 086, Telangana *Corresponding Author: Siva Reddy Challa, Department of Pharmacology, Sri Indu Institute of Pharmacy, Ibrahimpatnam-501510, Ranga Reddy District, Andhra Pradesh, India. Email: sivareddypharma@gmail.com
Online published on 17 February, 2020. Abstract The study was aimed to evaluate the protective effects of alcoholic extracts of Strawberry and Blueberry fruits [AESF and AEBF] in acute pancreatitis in rats. Treatment groups received AESF and AEBF at doses of 200 and 400 mg/kg for 7 days with prior injections of L-arginine on 5th day. Biochemical parameters were estimated in serum and pancreatic tissue samples. Histopathological studies and DNA fragmentation assay were carried out in isolated pancreatic tissue. The results of the study indicated that treatment of AESF and AEBF exhibited a significant dose dependent protective effect. Upon the treatment, anti-oxidant enzymes were significantly (*p<0.05) increased. Biochemical results were correlated with the histopathological findings. In addition, the DNA fragmentation assay showed an intact DNA in pancreatic cells of treated groups. In conclusion, berry fruit extracts exerted a potential protective effect against L-arginine induced damage in rat pancreas, at least in part, due to its antioxidant properties. Top Keywords Amylase, Blueberry, L-Arginine, Lipase, Oxygen free radicals, Pancreatitis, Strawberry. Top |