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Indian Journal of Animal Research
Year : 2019, Volume : 53, Issue : 1
First page : ( 77) Last page : ( 83)
Print ISSN : 0367-6722. Online ISSN : 0976-0555.
Article DOI : 10.18805/ijar.B-821

Effects of dietary Bacillus amyloliquefaciens on mucosal immunity, cecal volatile fatty acids and microbial diversity in broiler chickens

Cao G. T.1,2, Zhan X.A.2, Zhang L.L.1, Zeng X.F.3, Chen A.G.2, Yang C.M.1,*

College of Animal Science and Technology, Zhejiang A and F University, Hangzhou, China

1College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, China

2College of Animal Sciences, Zhejiang University, Hangzhou, China

3Zhejiang Huijia Biological Technology Ltd., Anji, China

*Corresponding author's e-mail: yangcaimei2012@163.com

Online published on 26 February, 2019.

Abstract

This trial was conducted to investigate the adhesion of Bacillus amyloliquefaciens to Caco-2 cells, the effects of Bacillus amyloliquefaciens on the mucosal immunity, cecal volatile fatty acids and microbial diversity in broiler chickens. Three hundred and sixty 1-d-old Ross 308 chicks were randomly divided into 3 dietary treatments groups, which birds fed with basal diet, basal diet supplemented with colistine sulfate, and basal diet supplemented with Bacillus amyloliquefaciens. Polymerase chain reaction denaturing gradient gel electrophoresis was used to analyze the change of cecal microflora, and Gas Chromatography was used to analyze the cecal volatile fatty acids. Data showed that: 1) Bacillus amyloliquefaciens had a good adhesion ability to epithelial cells; 2) the supplementation of Bacillus amyloliquefaciens significantly increased the concentration of ileal mucosal secretory IgA and interleukin 6, decreased (P<0.05) the concentration of tumor necrosis factor-α 3) resulted in the change of cecal microbial community, higher levels of acetic acid, methylacetic and isovaleric acid in the birds. Thus, we considered that Bacillus amyloliquefaciens enhanced the mucosal immunity, increased the cecal concentration of major volatile fatty acids and the diversity microflora community in broilers.

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Keywords

Bacillus amyloliquefaciens, Broiler chickens, Cecal microflora, Mucosal immunity, Volatile fatty acids.

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