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Indian Journal of Animal Research
Year : 2018, Volume : 52, Issue : 6
First page : ( 843) Last page : ( 850)
Print ISSN : 0367-6722. Online ISSN : 0976-0555.
Article DOI : 10.18805/ijar.v0iOF.7828

Myostatin silencing effect on basic helix-loop-helix transcription factors in caprine foetal fibroblast cells

Borah1 Biswajyoti, Singh Ajit Pratap*, Gogoi Hamen, Phukan Amlan Jyoti, Sarkhel Bikash Chandra

Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur-482004, Madhya Pradesh, India

1Department Animal Biotechnology, College of Veterinary Science, AAU, Khanapara, Guwahati, India

*Corresponding author's e-mail: borah.drbiswajyoti@gmail.com

Online published on 18 July, 2018.

Abstract

Transgenic food animal production is one of the potential and need oriented research to mitigate the food crises of the world. In vitro gene silenced animal cells and making use of these cells for transgenesis one of the suitable way to produce productive animals. Myostatin is a negative regulator of muscle growth, has the potential to increase the muscle mass upon its silencing. Four Hush 29-mer anti-myostatin (MSTN) shRNA constructs were checked for myostatin gene silencing in caprine foetal fibroblast cells and its subsequent effect on basic helix-loop-helix (bHLH) transcription factors. These factors are necessary for the terminal differentiation, proliferation, and homeostasis of muscle development. Different shRNA constructs displayed 55.1 to 91.5% (p < 0.01) of myostatin silencing in caprine foetal fibroblast cells and upregulation of myogenic gene. Upregulation of 7.97 to 111.67% for MyoD, 77.0% to 319.47% for myogenin, 16.67% to 138.0% for Myf5 were observed. The Pearson correlation established a negative correlation between myostatin and genes under study. Result suggests that knockdown of MSTN a potential approach to improve caprine musculatures.

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Keywords

Basic helix-loop-helix, Caprine, Foetal Fibroblast, yostatin, Silencing, Transcription factors.

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