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Indian Journal of Animal Research
Year : 2017, Volume : 51, Issue : 5
First page : ( 817) Last page : ( 820)
Print ISSN : 0367-6722. Online ISSN : 0976-0555.
Article DOI : 10.18805/ijar.v0iOF.7609

Polymorphisms of the Myostatin gene (MSTN) and its association with growth traits in Bali cattle

Nugroho Hary*, Busono Woro, Maylinda Sucik

Departement of Animal Production, Faculty of Animal Husbandry, University of Brawijaya, Malang, Indonesia

*Corresponding author's e-mail: nugroho_hy@yahoo.com

Online published on 4 November, 2017.

Abstract

This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5'-CCCTACAGAGGCCACTTCAA-3 ’and (R) 5'-CTCGCTGTTCTCATTCAGATC-3 ’, sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection.

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Keywords

Bali cattle, Growth traits, Myostatin gene, Polymorphism, PCR-RFLP.

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