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Indian Journal of Animal Research
Year : 2009, Volume : 43, Issue : 1
First page : ( 37) Last page : ( 40)
Print ISSN : 0367-6722.

Evaluation of seed germination test for early detection of pregnancy in cows1

Krishna S.V. Rao*, Veena T.

Department of Veterinary Physiology, Veterinary College, KVAFSU, Bangalore-560 024, India.

*Corresponding Address: 227, first floor, 5th cross, 8th main, Hebbal first stage, Mysore-570 016, India.

1Part of M.V.Sc., thesis submitted to KVAFSU, Bangalore-560 024, India by first author.


The urine samples collected from 40 crossbred cows/heifers during natural micturition early in the morning on day 14, 21, 28, 35 and 45 after artificial insemination (AI) were subjected to seed germination test using wheat seeds and green gram. A significant inhibition of seed germination after 48 hours and shoot growth after four days in both types of seeds, discoloration of either seeds or the germination fluid or both after 48 hours and deepening thereafter when compared to control groups were taken as criteria for declaring positive for true pregnancy. The results indicated that such observations could be noticed for the first time in 68 per cent of the test pregnant group, especially 28 days post AI. But the efficacies for true pregnancy detection on days 35 and 45 were 100 per cent, each. This test may serve as a simple, non invasive, dependable, user friendly, economical and door step technique for early pregnancy detection in cows, to the field veterinarians and farmers equally.


Key words

Seed germination test, Pregnancy detection.


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