Isolation and Characterization of Active Compound from Fruits of Medicinal Plant Spondias pinnata Kurz. Kandali R1, Konwar B K2 1Department of Biochemistry and Agricultural Chemistry, BN College of Agriculture, Assam Agricultural University, Biswanath Chariali, Sonitpur, Assam-784176, India. ranjan_kandali@yahoo.com 2Department of Molecular Biology and Biotechnology, Tezpur University, Sonitpur, Assam-784028, India. Abstract Methanolic extract of fruits of Spondias pinnata Kurz. (Anacardiaceae) was partitioned sequentially with petroleum ether, ethyl acetate, chloroform and butanol. The chloroform fraction possessed antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphyllococcus aureus and Candida albicans. Fractionation of the bioactive chloroform extract afforded SP1 as the major bioactive compound showing activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Candida albicans. However, it did not show activity against Klebsiella pneumoniae. On the basis of IR, HRFABMS, 1H NMR, 13C NMR spectroscopic data, the structure of the compound was determined to be ‘3 â-hydroxyolea-12-en-28-oic acid’ commonly known as ‘oleanolic acid’. Top Key words Spondias pinnata, active compound, oleanolic acid. Top |