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Indian Journal of Agricultural Biochemistry
Year : 2009, Volume : 22, Issue : 2
First page : ( 78) Last page : ( 82)
Print ISSN : 0970-6399. Online ISSN : 0974-4479.

Capsaicin Production in Cell Suspension Cultures Derived from Placenta of Capsicum annuum L. Fruit

Pandhair Varindra1,*, Gosal S S1,2

1Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India.

2School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India.

*Author for correspondence: Email: varindra_pandhair@hotmail.com

Received:  4  March,  2009; Accepted:  21  July,  2009.

Abstract

Capsaicin production by cell suspension cultures derived from placenta of Capsicum annuum L. var Punjab Lal fruits were studied by feeding precursors, elicitors and stressing media. Out of the various nutrient stresses tried, 100% nitrogen stress was most effective in increasing capsaicin in cell suspension cultures by 9.8 fold of that in control after 15 days of subculturing (DOS). Addition of 4mM tyrosine to 100% P and 100% K stress led to 8.4 folds and 7.5 folds higher capsaicin production as compared to control, respectively. Out of various precursors tried, 4mM coumaric acid was most effective followed by 5mM ferulic acid and 4mM vanillin which showed 6.3, 4.7 and 2.4 fold increase, respectively. Elicitor phycocyanin (0.25%) increased capsaicin upto 2.8 folds, whereas sinapic acid (0.05mM) was most effective and increased capsaicin upto 8.9 folds in the cell suspension cultures. Salicylic acid (2mM) and curdlan (0.0625%) had no effect but when 4mM tyrosine was supplemented along with curdlan (0.0625%), capsaicin accumulation increased upto 8.7 fold with parallel increase in phenylalanine ammonialyase activity.

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Key words

Cell suspension cultures, capsaicin, placenta, precursors, stress, elicitors.

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