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Current Trends in Biotechnology and Pharmacy
Year : 2015, Volume : 9, Issue : 3
First page : ( 222) Last page : ( 227)
Print ISSN : 0973-8916. Online ISSN : 2230-7303.

Phenotypic and Genotypic Characterization of Indigenous Lactobacillus Species from Diverse Niches of India

Thakur Kiran1,*, Kumar Narendra1, Nanda Dhiraj Kumar1, Tomar Sudhir Kumar1

National Collection of Dairy Cultures, Karnal, Haryana, India-132001

1Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India-132001

*For Correspondence-sudhirndri@gmail.com

Online published on 16 December, 2015.

Abstract

Lactobacilli are well reckoned for their technological and probiotic significance and their exercise in dairy products for trade and industry. The present study was carried out to isolate and characterize Lactobacilli from various dairy and non dairy sources of Indian origin. The isolates were subjected to phenotypic and genotypic characterizations followed by partial 16S rDNA sequencing. As many as 40 isolates were identified as Lactobacilli and among them 14 were identified as L.plantarum, 5 as L. fermentum, 5 as L. delbrueckii subsp. bulgaricus, 4 L. brevis, 2 L. pentosus and 1 isolate as L. mucosae. The great diversity was found with the place as well as source of collection.

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Keywords

Lactobacilli, human feces, curd and bamboo shoots.

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