Antiproliferative and antiangiogenic effects of partially purified Luffa acutangula fruit extracts on human lung adenocarcinoma epithelial cell line (A-549) Reddy Boreddy Purushotham, Goud R. Kannaiah, Mohan S. Venkata*, Sarma P. N. Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, 500 607, Andhra Pradesh, India * For correspondence - vmohan_s@yahoo.com.
Abstract The present study was undertaken to evaluate the possibility of Luffa acutangula (cucurbitaceae family) fruit as a potential anticancer agent by examining antiproliferative and antiangiogenic activities. Fruit methanolic extract showed significant antiproliferative activity (IC50, 131.63±2.31 μg/ml) on human lung adenocarcinoma epithelial cell line (A-549). The extract was partially purified by chromatography. Fraction F2–3 showed most potent antiproliferative activity (IC-50, 7.61±1.03 μg/ml) and was further evaluated for antiangiogenic activity by evaluating vascular endothelial growth factor (VEGF), matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-2 (MMP-9) as in-vitro, chick chorioallantoic membrane (CAM) as an in-vivo model for VEGF. VEGF and both MMP protein expressions were significantly inhibited in F2–3 treated A-549 cells compared to control cells (VEGF: 4.36±0.47 and 14±0.75 pg/ml, MMP-2: 10.17±1.3 and 20.28±1.68, MMP-9: 12.93±1.70 and 21.12±2.12 ng/ml, respectively). Egg chorioallantoic membrane treated with F2–3 fraction (10 μg) showed clear avascular zones compared to phosphate buffered saline (PBS) treated eggs. In conclusion, our data provides a scientific proof for Luffa acutangula as a potential antitumor agent. Top Keywords Vascular endothelial growth factor (VEGF), Matrix metalloproteinases MMP), Extracellular matrix (ECM), Chick chorioallantoic membrane (CAM) model. Top |