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Current Trends in Biotechnology and Pharmacy
Year : 2008, Volume : 2, Issue : 2
First page : ( 334) Last page : ( 340)
Print ISSN : 0973-8916. Online ISSN : 2230-7303.

Studies on Designing, Construction, Cloning and Expression of a novel synthetic antimicrobial peptide

Babu P Jawahar, Pulicherla KK, Rekha VPB.1, Nelson R2, Rao KRS Sambasiva*

Center for Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India

1Department of Biotechnology, JJ College of Arts and Science, Puddukottai-622 507, Tamil Nadu, India

2Department of Botany, Government Arts College, Ariyalur-621713, Tamil Nadu, India

*For correspondence –krssrao@yahoo.com

Abstract

In the present study a 9 amino acid synthetic anti microbial peptide was designed by using the bioinformatics tools viz., ProtParam, Hierarchical Neural Network, Anti microbial peptide database. The designed peptide was back translated to core nucleotide sequence and the gene for the peptide was constructed by over lapping PCR methodology. Synthesized gene was cloned into pRSET-A vector and further the peptide was expressed in E.coli host systems-BL (21) DE3 and GJ1158. A comparative study was performed and identified GJ1158 as the best expression system and the anti bacterial activity was analyzed on various Gram +Ve and Gram – Ve bacteria.

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Keywords

Anti Microbial Peptide, Synthetic Peptides, Cationic peptide.

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