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Current Trends in Biotechnology and Pharmacy
Year : 2008, Volume : 2, Issue : 1
First page : ( 192) Last page : ( 197)
Print ISSN : 0973-8916. Online ISSN : 2230-7303.

Screening of Dairy Bulls for Weaver Syndrome in India

Patel R.K.1,*, Singh K.M.1, Soni K.J.1, Chauhan J.B.1, Rao K.R.S. Sambasiva2

1R & D-Biotechnology, National Dairy Development Board, Anand-388 001, Gujarat, India

2Centre for Biotechnology, Acharya Nagarjuna University, Nagarjunanagar-522 510, Tamil Nadu, India

*For correspondence -rkpatel46@yahoo.com

Abstract

Progressive Degenerative Myelo Encephalopathy (PDME) or the Weaver's Syndrome is an autosomal recessively inherited neurological disease mainly found in Brown Swiss breed. In the present study an attempt was made to find out the prevalence of different alleles of the microsatellite marker, TGLA116 in exotic, indigenous and their crossbred cattle and different breeds of buffalo population in India. Blood samples were collected from a panel of 214 cattle and 86 buffalo of different breeds belonging to various sperm stations across the country for Genomic DNA extraction. The PCR amplified products generated with TGLA116 primers were used for microsatellite analysis using sequencing polyacrylamide gel (PAGE). Homozygous and heterozygous alleles were observed in polyacrylamide gel, occasionally along with higher molecular weight non-specific amplificons. Alleles were sized by using a 25bp DNA hyper ladder, 82 bp-digested product of Citrullinaemia and INCHWORM programme. The homozygous and heterozygous alleles of various sizes observed in different breeds of cattle and buffaloes ranged from 76 to 87 bp and 76 to 91 bp respectively. Out of 300 animals screened, 4 HF, 4 Gir cattle and one Murrah buffalo exhibited homozygous allele of 83 bp that could be related to weaver disease. But none of these adult cattle and buffalo bulls showed any symptoms of the weaver disease, indicating that presence of 83 bp homozygous allele is not related to the disease. One of the heterozygous alleles with 83 bp was also observed in 20 cattle breeds and 1 buffalo. However it is difficult to call them carriers, as 83 bp homozygous alleles could not show the symptoms of the disease in 8 cattle and 1 buffalo were phenotypically normal even after attaining the age of puberty. In view of the above the laboratory has discontinued screening dairy bulls for weaver syndrome.

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Keywords

Weaver disease, PDME, DNA, PCR, PAGE, alleles, microsatellite.

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