Bio Science Research Bulletin
Year : 2012, Volume : 28, Issue : 1
First page : ( 49) Last page : ( 62)
Print ISSN : 0970-0889. Online ISSN : 2320-3161.

Protective effect of vitamin B complex against renotoxic tissue damage induced by diclofenac sodium in rats

Ayaz Najla O.

Department of Biochemistry, Sciences Faculty For Girl's, King Abdulaziz University, Jeddah, Saudi Arabia

Online published on 11 January, 2013.

Abstract

The aim of this study was to investigate the protective role of vitamin B complex (vit B₁, B₆ and B₁₂) in ameliorating the metabolic abnormalities and oxidative damage induced by diclofenac sodium (Voltaren) toxicity in rat kidneys. Diclofenac sodium was administered intra-peritoneally at either 1.5 and 3 mg/Kg body weight for 14 consecutive days. The results revealed that co-administration of vitamin B complex (1.6 mg B₁ 1.6 mg B₆ and 16.7 mg B₁₂/Kg body weight, i.p.) daily for 3 weeks to rats intoxicated by either of the two doses markedly could protect the damaging effect of diclofenac on rat kidneys which as evidenced by marked reduced level of serum urea, uric acid and creatinine in relation to diclofenac intoxicated rats. The B complex also could markedly attenuate the decreases in blood Hemoglobin (Hb) level as well as in the renal antioxidant enzymes namely, glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PDH) and catalase (CAT) compared with diclofenac intoxicated rats. The current result also showed that the administration of vitamin B complex along with diclofenac injection to rats successfully modulated the metabolic disorder caused by dlclofenac toxicity which documented by its beneficial action in regulating the increases in serum glucose, serum lipid profiles; triglycerides (TG), total cholesterol (TCh) and low density lipoprotein (LDL) as well as the decrease in the high density lipoprotein (HDL).

These results support the use of vitamin B complex as protective agent against diclofenac toxic side effect induced renal tissue damage.

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Keywords

Diclofenac sodium, Vitamin B complex, Antioxidant enzymes, Metabolic disorder.

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