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Rapid HPLC Method for Determination of Cholesterol using C18 Column Yadav Nutan B.*, Kulkarni M. V.* Zoology Department, Agharkar Research Institute, Pune-411 004 *Biochemistry Division Department of Chemistry, University of Pune, Pune. Online published on 17 January, 2012. Abstract A method for quantitative estimation of cholesterol using C18 reversed phase column and evaporative light scattering detector was developed by optimizing technical parameters, such as column oven temperature (450C), mobile phase composition (acetonitrile - isopropanol, 60: 40), mobile phase flow rate (1.5 ml/min), nebulizer temperature (550C) and evaporator temperature (850C). The calibration curve was established with 2.5 - 160 µ/ml standard cholesterol with correlation coefficient (r2) value of 0.99. Top Keywords Cholesterol, HPLC, ELSD. Top | |
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