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BIOINFOLET - A Quarterly Journal of Life Sciences
Year : 2019, Volume : 16, Issue : 3
First page : ( 146) Last page : ( 153)
Print ISSN : 0973-1431. Online ISSN : 0976-4755.

Detection of antibiotic genes from endophytic Pseudomonas fluorescens of cotton

Devi P. Ahila

Plant Pathology Unit, National Pulses Research Center, Vamban, Pudukkottai Dt

Online published on 14 November, 2019.

Abstract

The production of antibiotics, by endophytic Pseudomonas fluorescens was detected through thin layer chromatography (TLC) with the help of Rf values. Metabolites such as 2, 4-Diacetyylphloroglucinol DAPG (0.5), Phenazine (0.88), Pyrolnitrin (0.60), Pyoluteorin (0.94) and Pyocyanin (0.10) were detected with the Rf values as mentioned in the parenthesis. The genomic DNA of P. fluorescens was amplified with predicted fragment of DAPG (420bp), Phenazine (220 bp), Pyrrolnitrin (530 bp) and Pyoluteorin (359 bp)

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Keywords

Cotton, TLC, antibiotic genes, toxin assay.

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