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Asian Journal of Research in Pharmaceutical Science
Year : 2016, Volume : 6, Issue : 2
First page : ( 135) Last page : ( 138)
Print ISSN : 2231-5640. Online ISSN : 2231-5659.
Article DOI : 10.5958/2231-5659.2016.00019.9

UV Spectrophotometric Method Development and Validation of Fluticasone Propionate

Kulkarni Rajashri R.1,*, Phadtare Dipti G.2, Saudagar Ravindra B.2

1Department of Quality Assurance Techniques, R. G. Sapkal College of Pharmacy, Anjaneri, Nashik

2Department of Pharmaceutical Chemistry, R. G. Sapkal College of Pharmacy, Anjaneri, Nashik

*Corresponding Author E-mail: kulkarniraj1993@gmail.com

Online published on 21 June, 2016.

Abstract

Analytical methods development and validation play important roles in the discovery, development, and manufacture of pharmaceuticals. Simple, precise and accurate UV spectroscopic method has been developed and validated for estimation of Fluticasone propionate. It is a selective agonist at the glucocorticoid receptor. UV spectroscopic method which is based on measurement of absorption of UV light, the spectra of Fluticasone propionate in methanol showed maximum wavelength at 236nm and calibration curve were plotted over the concentrations ranging from 2-22ug/ml of Fluticasone propionate with correlation coefficient 0.9812 validation was performed as per ICH Q2 (R1) guidelines for linearity, accuracy, precision and recovery. The proposed method was validated.

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