Development and Validation of Spectrophotometric Method for Determination of Azelaic Acid Kadam T.V.1,*, Darekar A.B.1, Gondkar S.B.1, Saudagar R.B.2 1Department of Pharmaceutics, R.G. Sapkal College of Pharmacy, Anjaneri, Nashik-422213, Maharashtra, India 2Department of Pharmaceutical Chemistry, R.G. Sapkal College of Pharmacy, Anjaneri, Nashik-422213, Maharashtra, India *Corresponding Author E-mail: truptikadam91@gmail.com
Online published on 8 December, 2015. Abstract In these studies describes a simple, accurate, precise and cost effective UV-visible spectrophotometric method for the estimation of Azelaic acid in pure and pharmaceutical formulations. The method is based on the measurement of absorbance of Azelaic acid solution in Phosphate buffer pH 6.8 at 204nm in the wavelength range of 200-400nm. The method obeys Beer's Lambert's law in the selected concentration range10-50 μg/ml in selected media. The slope, intercept and correlation coefficient were also calculated. Results of percentage recovery study shows that the method was not affected by the presence of common excipients in tablets. The parameters like linearity, precision, accuracy, sensitivity study i.e. limit of detection and limit of quantitation were studied according to International Conference on Harmonization (ICH) guidelines. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantitation which proves suitability of proposed method for routine estimation of Azelaic acid in pure and pharmaceutical formulations. Top Keywords UV-visible spectrophotometer, method development, Azelaic acid. Top |