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Year : 2021, Volume : 11, Issue : 1
First page : ( 35) Last page : ( 40)
Print ISSN : 2231-5640. Online ISSN : 2231-5659. Published online : 2021  15.
Article DOI : 10.5958/2231-5659.2021.00006.0

Isolation, Identification, Characterization and Optimization of Amylase producing organisms from sacred groves of Mahesana District

Prajapati H. V.1*, Prajapati D. D.1, Bhatt S. A.2

1Dept. of Microbiology, Mahesana Urban Institute of Science, Ganpat University, Mahesana, India.

2Dept. of Life Science, Hemchandracharya North Gujarat University, Patan, India.

*Corresponding Author E-mail: Prajapati.dhaval64@gmail.com

Received:  03  ,  2020; Accepted:  03  October,  2020.

Abstract

Amylase is the most widely used Food, fermentation, Starch processing, textile and papermaking. This research was conducted to isolate and identify a local amylase producing bacteria from sacred groves soil and characterize, Bacteria from soil were primarily screened on starch agar medium and out of 49 isolates, 15 were found to be amylase producers, The isolates were screened based on their clear zone ratio, enzyme activities The best isolate demonstrated an enzyme activity of MH-31, MH-48 and MH-43, It was then identified by a combination of biochemical tests, morphological and microscopic characteristics along with 16S rRNA gene sequencing from all six isolate the optimization Temp-35°C, pH-7, Carbon source-Glucose, Nitrogen Source- Yeast extract, salt Concentraction: 3 this all condition were recoeded.

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Keywords

Amylase, Sacred Groves, Temperature, Carbon source, Nitrogen Source, Optimization.

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