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Asian Journal of Research in Chemistry
Year : 2016, Volume : 9, Issue : 8
First page : ( 369) Last page : ( 372)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2016.00056.0

UV Spectrophotometric Method Development and Validation of Benazepril Hydrochloride

Kokane Prajakta A.1,*, Bhairav Bhushan A.1, Saudagar Ravindra B.2

1Department of Quality Assurance Techniques, R. G. Sapkal College of Pharmacy, Anjaneri, Nashik

2Department of Pharmaceutical Chemistry, R. G. Sapkal College of Pharmacy, Anjaneri, Nashik

*Corresponding Author E-mail:

Online published on 18 October, 2016.

Abstract

Analytical methods development and validation play important roles in the discovery, development, and manufacture of pharmaceuticals. Simple, precise and accurate UV spectroscopic method has been developed and validated for estimation of Benazepril Hydrochloride. It is a angiotensin converting enzyme inhibitors. UV spectroscopic method which is based on measurement of absorption of UV light, the spectra of Benazepril Hydrochloride in methanol showed maximum wavelength at 237 nm and calibration curve were plotted over the concentrations ranging from 2-22ug/ml of Benazepril Hydrochloride with correlation coefficient 0.961 validation was performed as per ICH Q2 (R1) guidelines for linearity, accuracy, precision and recovery. The proposed method was validated.

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Keywords

Benazepril Hydrochloride, methanol, water, Co-solvency method, spectrophotometer and validation.

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