Validated RP-HPLC Method for the Simultaneous Estimation of Simvastatin and Niacin Akula Ganesh1,*, Venkatesh Bollaboina2, Sanjayraj K.2, Phanindra S.S.2, Dr. Jaswanth A.3 1Department of Pharmaceutical Chemistry, Procadence Institute of Pharmaceutical Sciences, Rimmanaguda, Gajwel, Medak (dt)-502312, Telangana, India 2Department of Pharmaceutical Analysis, Procadence Institute of Pharmaceutical Sciences, Rimmanaguda, Gajwel, Medak (Dt)-502312, Telangana, India 3Department of Pharmacology, Procadence Institute of Pharmaceutical Sciences, Rimmanaguda, Gajwel, Medak (Dt)-502312, Telangana, India *Corresponding Author E-mail: akulaganesh@gmail.com
Online published on 29 March, 2016. Abstract A simple, accurate, precise, rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of Simvastatin and Niacin in Pure and Tablet dosage forms. Inertsil ODS, RP-18 Column (250 x 4.6 mm ID, 5μ) was used with a mobile phase containing a mixture of Phosphate buffer pH 2.5, Methanol and Acetonitrile in the ratio of 45: 20: 35. The procedure was carried out at pH-3.5. The compounds were eluted at a flow rate of 1.0 ml/min. results were determined at 220 nm with fixed wavelength PDA detector. The linearity for Niacin was found between 75–175 μg/ml and between 3–7 μg/ml for Simvastatin. The retention times were found as 4.747 min and 2.970min for Niacin and Simvastatin respectively. The above method was validated in terms of System suitability, linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ), Robustness in accordance with ICH guidelines. The method was rapid, simple, economical and suitable for routine quality control analysis. Top Keywords Simvastatin, Niacin, Method development, Validation, RP-HPLC. Top |