Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Lornoxicam and Eperisone in their Synthetic Mixture Akhtar Jawed1,*, Prajapati Jatin2, Ahmad Shamim1, Mujahid Mohammad1, Elhassan Gamal Osman3 1Translam Institute of Pharmaceutical Education and Research, Meerut, Uttar Pradesh-250001 2Astra Life Care Pvt. Ltd, Ahmedabad 3Faculty of Pharmacy, Omdruman Islamic University, Khartoum, Sudan *Corresponding Author E-mail: jawed.pharmacy@gmail.com
Online published on 17 October, 2015. Abstract A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Lornoxicam (LXM) and Eperisone (EPE) by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 264.5 nm (zero cross point of LXM) was used for quantification of EPE and 254 nm (zero cross point of EPE) for quantification of LXM. The linearity was established over the concentration range of 2–24 μg/ml with correlation coefficient 0.9987 and 0.997, respectively. The mean % recoveries were found to be in the range of 99.69 and 99.13 for LXM and EPE respectively. The developed method has been validated as per ICH guidelines and successfully applied to the estimation of LXM and EPE in their Synthetic Mixture. Top |