Optimization of Enzymatic Synthesis of 3-O-β-D-Glucopyranoside Betulinic Acid by Novozyme-435 Abdu Hamisu1,3, Ahmad Faujan B. H.2,*, Basri M.1, Ismail Intan Safinar1, Rahman M.B. Abdul1 1Department of Chemistry, Faculty of Science, University Putra Malaysia, Malaysia 2Natural Product Laboratory, Faculty of Applied Sciences, Universiti Teknologi MARA, Malaysia 3Hussaini Adamu Federal Polytechnic, Kazaure, Jigawa State, Nigeria *Corresponding Author E-mail: faujanahmad@gmail.com
Online published on 28 August, 2014. Abstract The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69% and the predicted value was 89.05%. Top Keywords Enzymatic synthesis, 3-O-β-D-glucopyranoside betulinic acid, Novozyme-435 and optimization. Top |