A Validated Simple UV Spectrophotometric Method for the Estimation of Pitavastatin in bulk and Pharmaceutical Dosage Form Yunoos Mohammad*, Sowjanya M., Sushma B., Kumar K. Pavan Department of Pharmaceutical Analysis, Bapatla College of Pharmacy, Bapatla-5222101, Andhra Pradesh, India *Corresponding Author E-mail: yunoosvja@gmail.com
Online published on 28 August, 2014. Abstract A simple, accurate, rapid and sensitive UV spectrophotometric method has been developed for the estimation of Pitavastatin (PVT) in bulk as well as in Pharmaceutical dosage forms. Pitavastatin is used as Anti-hyperlipidemic agent and HMG-CoA reductase inhibitor. It is available in the market in tablet dosage forms with strength 1mg, 2mg and 4mg. The present research work involves the estimation of Pitavastatin using 0.1N HCL as solvent. An absorption maximum (λ max) was found to be 249.5nm. It obeys the Beer's law in the concentration range of 2–12μg/ml with a correlation coefficient value of 0.9996. Validation of the method has been carried out statistically as per ICH guidelines and the results were found to be satisfactory. The result of mean % recovery was found to be 99.83 ± 0.39% for Pitavastatin which shows that the method was not affected by the presence of common excipients in tablet dosage form. The % RSD for both inter-day and intra-day precision was found to be less than 2%. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.122 μg/mL and 0.371 μg/mL. The method was validated by determining its sensitivity, linearity, accuracy and precision which proves suitability of the developed method for the routine quality control analysis for the estimation of Pitavastatin in bulk and tablet dosage form Top Keywords Pitavastatin (PVT), Spectrophotometric method, Validation, ICH guidelines, tablet dosage form. Top |