Antifertility activity of Kaempferol-7-O-Glucoside isolate from Cassia nodosa Bunch
Rani Rachana1,*, Mishra Sudeep2
1Banasthali Vidyapith, Banasthali, Jaipur, India
2Govt. P. G. College, Satna, India
*Corresponding Author E-mail: firstname.lastname@example.org
Online published on 7 February, 2013.
Cassia species have been of keen interest in phytochemicals and pharmacological research due to their excellent medicinal values. Different classes of natural products, possessing potent physiological and pharmacological activities have been isolated from Cassia nodosa. These included anthracene derivatives, flavonoids and polysaccharides. Some of these compounds have been shown their antifertility activity. In this research article, we reported the isolation, purification of flavonoid from different parts of C. nodosa and their antifertility activity. A new flavonoid kaempferol7-O-glucoside was identified, characterized and it showed significant antifertility activity. The higher levels of total flavonoid were measured in flowers of C. nodosa (1.39 mg/gdw). Similarly higher levels of total qurecetin (F+B) were measured in flowers of C. nodosa (0.42 mg/gdw). Higher levels of total kaempferol (F+B) was measured in flowers of C. nodosa (0.68 mg/gdw). Similarly higher levels of kaempferol-7-O-glucoside were measured in flowers of C. nodosa (0.29 mg/gdw). Kaempferol-7-O-glucoside feeding to rats significantly reduced sperm concentration of testes and epididymisdes (P<0.001). The motility of the cauda epididymal sperm was also reduced significantly (P<0.001). The isolate kaempferol-7-O-glucoside reduced the fertility of male rats by 95%.
Cassia nodosa, Kaempferol, Quercetin, Kaempferol-7-O-glucoside, IR, NMR, Antifertility.