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Asian Journal of Research In Chemistry
Year : 2012, Volume : 5, Issue : 2
First page : ( 193) Last page : ( 196)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

HPTLC methods for quantification of gallic acid in Bhuvneshvara vati for routine quality control

Jain V.1,*, Daharwal S.J.1, Jain Tripti2, Saraf Swarnlata1, Saraf S.1

1University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010

2Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 010

*Corresponding author: vj_rsofiop@rediffmail.com

Online published on 7 February, 2013.


Bhuvneshvara vati is one of the popular Ayurvedic preparations, official in Ayurvedic formulary of India. The present study is an attempt to develop a fingerprint method for Bhuvneshvara vati HPTLC with Method using gallic acid as a standard. The gallic acid is a major content in all the five herbal ingredients of the formulation. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 3.10 ±0.41% w/w in emblica officinalis, 8.47±0.82% w/w in terminalia belerica, 4.63±0.49% w/w in Terminalia chebula, 1.82±0.73% w/w in Aegle marmelous and 0.26±0.65% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvneshvara vati BV-I, BV-II and BV-III, is found to be 2.61±0.468%, 2.59±0.567% and 2.63±0.392% w/w respectively with mean value 2.61±0.476%. The gallic acid content in all the three different batches is found to be in close proximities with each other.The results were comparable to marketed formulations.



HPTLC, Gallic acid, Bhuvneshvara vati, Ayurvedic formulation, Fingerprinting.


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