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Asian Journal of Research in Chemistry
Year : 2009, Volume : 2, Issue : 3
First page : ( 306) Last page : ( 309)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

HPTLC estimation of Paracetamol, Diclofenac Sodium and Chlorzoxazone in Tablet dosage form

Pawar Smita J*, Kale Amol P, Amrutkar Manoj P, Jagade Jyotsna J, Pore Nikhil S, Bhosale Ashok V

PDEA's Seth Govind Raghunath Sable College of Pharmacy, Saswad, Pune-412301

*Corresponding Author E-mail: smitapawar4477@yahoo.co.in

Online published on 13 March, 2013.

Abstract

A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of Paracetamol, Diclofenac Sodium and Chlorzoxazone in tablets. The drugs were extracted from (CIP-ZOX). Various aliquots of this sample solution were spotted automatically by means of Camag (Muttenz; Switzerland) Linomat V sample applicator on Merck HPTLC plates (0.2mm thickness) precoated with silica gel 60 F254 on aluminium sheet as stationary phase prewashed with methanol using Chloroform: Methanol: Ammonia (20:5:0.2v/v/v) as mobile phase. The spots were scanned at λ=254nm using Camag TLC scanner 3. The Rf values of Paracetamol, Diclofenac Sodium and Chlorzoxazone were found to be 0.53, 0.27, and 0.68 respectively. Calibration curves were linear in range of 1000-5000ng per spot. The limit of detection (LOD) and quantitation (LOQ) for Paracetamol, Diclofenac sodium and Chlorzoxazone were found to be 100, 100, 500 and 300, 300, 1500ng per spot respectively. The suitability of this method for quantitative determination of compounds was proved by validation in accordance with requirements of pharmaceutical regulatory standards. The proposed method is valid, simple, sensitive and accurate. Therefore this method can be applied for routine analysis of these drugs in tablet formulation.

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Keywords

Paracetamol, Diclofenac sodium, Chlorzoxazone, HPTLC, Pharmaceutical dosage form.

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