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Asian Journal of Research in Chemistry
Year : 2009, Volume : 2, Issue : 2
First page : ( 131) Last page : ( 134)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

HPTLC Method for Quantitative Determination of Gallic Acid in Hydroalcoholic Extract of Dried Flowers of Nymphaea Stellata Willd.

Rakesh Sachin U*, Salunkhe VR, Dhabale PN, Burade KB

Govt. College of Pharmacy, Vidyanagar, Tal. Karad-415 124, Dist. Satara, M. S., India

*Corresponding Author E-mail: sachinrakesh@rediffmail.com

Online published on 13 March, 2013.


A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of gallic acid in the dried flowers of Nymphaea stellata willd. The hydroalcoholic extract of N. stellata was chromatographed on silica gel 60 F254 plates with chloroform: ethyl acetate: formic acid, 7.5: 6: 0.5 (v/v/v), as mobile phase. Detection and quantitation were performed by densitrometric scanning at A= 292 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of gallic acid was found to be 98.33%. The proposed HPTLC method provide a good resolution of gallic acid from other constituents present in hydroalcoholic extract of dried flowers of N. stellata and can be used for quantitation of gallic acid present in extract. The method is rapid, simple and precise.



HPTLC, gallic acid, Nymphaea stellata willd.


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