Condensation Methods for the Determination of Methoxamine Hydro Chloride in Pure and Pharmaceutical Formulations Acharyulu M.L.N.1, Associate Professor, Rao P.V.S.R. Mohana2,*, Research Scholar, Ramakoti I. Siva3, Assistant Professor, Jayarangarao P.4, Assistant Professor 1Centurion University of Technology and Management, Andhra Pradesh- 530017, India 2Department of Engineering Chemistry, A.U. College of Engineering (A), Visakhapatnam - 530003, A.P; India 3Department of Chemistry, Centurion University of Technology and Management, Odisha- 761211, India 4Baba Institute of Technology and Sciences, Visakhapatnam, A.P.India. *Corresponding Author E-mail: acharyulu@cutmap.ac.in
Abstract Two visible spectrophotometric methods were developed Aand B for the determination of Darunavir in pure and pharmaceutical formulations. The methods are based on condensation reaction with PDAB (Method-A) and ONB (Method-B) in presence of acidic medium with the primary amine group in DNV. The coloured products exhibit absorption Vax at 639 nm and 452nm for methods A and B respectively. Regression analysis of Beer- Lambert plots showed good correlation in the concentration ranges 10-60μg/ml, 50-300μg/ml, correlation co-efficients are 0.9983, 0.9989; Sandell’s sensitivities are 9.9833 x 10-33.0456 x 10-2 (1 mole cm-1); and molar absorptivity values are 5.4857 x 1041.7981x 104 (μg cm-2) for methods-Aand B respectively. The proposed methods are applied to commercial availabel formulations and the results are statistically compared with those obtained by the UV reference method and validated by recovery studies. The results are found satisfactory and reproducible. These methods are applied successfully for the estimation of the DNV in the presence of other ingredients that are usually present in formulations. These methods offer the advantages of rapidity, simplicity and sensitivity and low cost without the need for expensive instrumentation and reagents. Top Keywords Condensation, PDAB, ONB, Regression Analysis. Top |