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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 3
First page : ( 228) Last page : ( 232)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00044.9

Development of Validation of Zero order, First-order, Second-order, UV-Spectrophotometry Methods using AUC Technique for Quantitative Estimation of Linagliptin in Bulk Material and Tablets

Mr. Patil Chetan. V.1,*, Mr. Patil Paresh A.2

1R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (MS) 425405 India

2Ahinsa Institute of Pharmacy, Dondaicha, Shindkheda, Dhule. (MS) 425408 India

*Corresponding Author E-mail: rcp.pareshpatil@gmail.com

Online published on 10 August, 2020.


The present study deals with development and validation of a simple, rapid, sensitive and economic UV Spectrophotometry method Using AUC technique for Quantitative estimation of Linagliptin bulk material and tablet dosage form.

Materials and Methods

In method A, B and Ca stock standard solution was prepared by dissolving 10 mg of Linagliptin in 100 mL of 10% v/v methanol to obtain a concentration of 100 μg/mL. After suitable dilution, 10 μg/mL of Linagliptin was prepared and scanned in the UV-visible range 400–200 nm; Linagliptin showed a maximum absorbance at 295 nm. In Method A, Area Under Curve, (Auc)of the zero-order spectrum was recorded between 280 and 307 nm. While, in Method B, C, zero-order spectra were derivatized into first and second-order, and the AUC was recorded between 302 and 317 nm, and 290 and 303 nm,


In Method A, B and C, linagliptin followed linearity in the concentration range of 4–24 μg/mL (r2>0.999).


The amount of drug estimated for this methods are in good accord with labeled claim. The % RSD value in recovery, precision, and ruggedness studies are found to be not more than 2 indicated that the method is accurate and precise.



Linagliptin, U.V Spectroscopy, Derivative, Methods, Area Uder Curve, (AUC).


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