Development of Validation of Zero order, First-order, Second-order, UV-Spectrophotometry Methods using AUC Technique for Quantitative Estimation of Linagliptin in Bulk Material and Tablets
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The present study deals with development and validation of a simple, rapid, sensitive and economic UV Spectrophotometry method Using AUC technique for Quantitative estimation of Linagliptin bulk material and tablet dosage form.
Materials and Methods
In method A, B and Ca stock standard solution was prepared by dissolving 10 mg of Linagliptin in 100 mL of 10% v/v methanol to obtain a concentration of 100 μg/mL. After suitable dilution, 10 μg/mL of Linagliptin was prepared and scanned in the UV-visible range 400–200 nm; Linagliptin showed a maximum absorbance at 295 nm. In Method A, Area Under Curve, (Auc)of the zero-order spectrum was recorded between 280 and 307 nm. While, in Method B, C, zero-order spectra were derivatized into first and second-order, and the AUC was recorded between 302 and 317 nm, and 290 and 303 nm,
In Method A, B and C, linagliptin followed linearity in the concentration range of 4–24 μg/mL (r2>0.999).
The amount of drug estimated for this methods are in good accord with labeled claim. The % RSD value in recovery, precision, and ruggedness studies are found to be not more than 2 indicated that the method is accurate and precise.
Linagliptin, U.V Spectroscopy, Derivative, Methods, Area Uder Curve, (AUC).