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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 3
First page : ( 190) Last page : ( 197)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00037.1

Phytochemical screening and Identification of Polyphenols, Evaluation of Antioxidant activity and study of Biological properties of extract Silybum marianum (L.)

Guemari Fathi, Laouini Salah Eddine, Rebiai Abdelkrim*, Bouafia Abderrhmane

University of Echahid Hamma Lakhdar, El Oued-39000, Algeria

*Corresponding Author E-mail: abdelrahmanebouafia@gmail.com

Online published on 10 August, 2020.

Abstract

In this study, we were devoted to study this plant Silybum marianum (L.) we divided it into four parts of leaves, twigs, flowers, and grains. Among the criteria that led to the selection of this plant, is the geographical origin and traditional use of this plant, which affects the natural composition of the plant, our research focuses on the identification of phenolic compounds (polyphenols, flavonoids, and flavanol), evaluation of antioxidant and activity antimicrobial For the four samples of plant Silybum marianum (L.).The determination of the total polyphenols (TPC) by the method of Singleton and Ross using the Folin-Ciocalteu reagent, total flavonoid (TFC) by the method aluminum chloride method Ordonnez, and The estimation of the total flavanol content in the various extracts is carried out contained is carried out by the method of Mbaebie et al, The total antioxidant capacity (CAT) of the extracts is evaluated by the phosphomolybdenum method, DPPH test We use (2,2diphenyl-1-picrylhydrazyl), The determination of antioxidant capacity by the FRAP method is carried out as follows The FRAP method is basedon the present ferric iron (Fe3+) reduction reaction in the K3Fe (CN)6 ferrous iron (Fe2+) complex by an antioxidant, The results of the separation by high performance liquid chromatography HPLC. Phenol content and flavonoid content and flavanol content of the four plant samples varied and phenol content showed More effective for antioxidant activity and flavonoid content showed More effective for antimicrobial.

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Keywords

Silybum marianum (L.), Extraction, Bioactive compound, Polyphenols, Antioxidant, Antibacterial.

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