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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 3
First page : ( 157) Last page : ( 162)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00031.0

Preparation and Optimization of Keratin from Sheep wool using response Surface Methodology

Seghir Bachir Ben1,*, Amor Iham Ben2, Beki Ahlam2, Souici Imane2, Hemmamia Hadia3, Soumeia Zeghoud3, Laouini Salah Eddine2, Rebiai Abdelkrim3

1Laboratory of Industrials Analysis and Materials Engineering, University 8 May 1945 Guelma, BP 401, Guelma-24000, Algeria

2Department of Process Engineering, Faculty of Technology, University Hamma Lakhdar El Oued, BP 789, El-Oued39000, Algeria

3Laboratory Valorisation and Technology of Saharan Resources (VTRS). University of El Oued, BP 789 El-Oued (39000), Algeria

*Corresponding Author E-mail: bbachir39@gmail.com

Online published on 10 August, 2020.


The wool consists of around 90% keratin, an essential ingredient in cosmetics, shampoos, and hair care creams. The goal of this study was to determine the optimum conditions for keratin from wool extraction. Keratin isolation using different agents for sulfitolysis has been studied using statistical experimental method. In the extraction procedure, concentration Thiourea (CH4N2S), temperature and time reaction were analysed. The optimization yield (%) of keratin was achieved at obtained corresponds to the concentration Thiourea of x1= 8.6%, the tempera-ture of x2= 65.50°C and the reaction time of x3= 2 h, using response surface methodology (RSM). In addition, because of its good the model of experiment was significant with p<0.05 and adj R2 = 0.9991 with a good yield of keratin.



Wool, Keratin, Sulfitolysis, Optimization, Response Surface.


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