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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 2
First page : ( 117) Last page : ( 122)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00024.3

Simultaneous Spectrophotometric Estimation of Rifampicin, Isoniazid and Pyrazinamide in their Pharmaceutical Dosage Form

Khawas Sourav, Parui Sampurna, Dey Suddhasattya, Mondal Sudip Kr., Sarkar Saptarshy*

Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur-713206, West Bengal, India

*Corresponding Author E-mail: saptarshysarkar@gmail.com

Online published on 7 April, 2020.

Abstract

A simple, accurate, precise, reproducible and economical UV spectrophotometric method was developed and validated for the estimation of Isoniazid (INH), Rifampicin (RIF) and Pyrazinamide (PYZ) in their bulk and pharmaceutical dosage form. First order derivative spectrophotometric method was used. Wavelength for Isoniazid is 721nm (zero crossing point of Rifampicin and Pyrazinamide), for Rifampicin is 322nm (zero crossing point of Isoniazid and Pyrazinamide) and for Pyrazinamide it is 256nm (zero crossing point of Isoniazid and Rifampicin). Beer Lambert's law was obeyed within the concentration range of 0.75–3.75μg/ml for Isoniazid, 1.5–7.5μg/ml for Rifampicin and 4–12μg/ml for Pyrazinamide. The proposed method was been validated statistically as per the ICH guidelines for linearity, accuracy, precision, specificity, LOD and LOQ. The method developed and validated successfully for the quantitative analysis of Isoniazid, Rifampicin and Pyrazinamide in bulk and dosage form.

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Keywords

Isoniazid, Rifampicin, Pyrazinamide, validation, UV Spectroscopy.

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