Förster Resonance Energy Transfer Investigation between PPO and Coumarin 151 Dyes and Docking studies with Prolyl-tRNA Synthetase Conformers Mansuri Mohmedyasin F.1, Rathod Shravan B.2,* 1Department of Microbiology, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India 2Department of Chemistry, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India *Corresponding Author E-mail: shravanathorizon93@gmail.com
Online published on 19 December, 2019. Abstract The Fluorescence Resonance Energy Transfer (FRET) between 2,5-diphenyl-1,3-oxazole (PPO) and 7-amino-4-(trifluoromethyl) chromen-2-one (Coumarin 151) dyes was investigated. In these studies, PPO behaves as a donor and Coumarin 151 as an acceptor. All the measurements were carried out in DMSO solvent. The quantum yield of the donor was found 0.48 using tryptophan as a standard at 280 nm excitation wavelength. Energy transfer was confirmed by excitation spectra, steady-state fluorescence quenching, and Time-correlated single photon counting measurements. Measured Förster distance (R0) and FRET efficiency (E) of the system are observed 33 Å and 0.24 respectively. Binding energies of the donor, acceptor and their derivatives with two conformers of Prolyl-tRNA synthetase (PDB: 4TWA, 4YDQ) are found different in magnitudes by docking studies. Top Keywords FRET, Energy transfer, Excitation, Binding energy, Docking. Top |