New High Performance Liquid Chromatographic Method for Determination of Vilazodone HCl in Pharmaceutical Dosage Form Yadav Nita1,*, Anju G2 1Department of Pharmacy, SRMSCET, Bareilly, Uttar Pradesh, India 2B. N. Institute of Pharmaceutical Sciences, Udaipur, RAJ, India *Corresponding Author E-mail: raj_neetu78@rediffmail.com
Online published on 2 June, 2018. Abstract A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Vilazodone in Tablet dosage form by using a C8 Chromasil column (250 × 4.6 mm, 5μm particle size), with mobile phase containing mixture of phosphate buffer and methanol in ratio of 30: 70, v/v with pH adusted to 5 with orthophosphoric acid was used. The flow rate was 1 ml/min and the effluents were monitored at 232 nm. The retention time was 6.008 min. The detector response was linear in the concentration of 4–20 mcg/ml. The respective linear regression equation being y = 66733x-4974. The limit of detection and limit of quantification was 0.418and 1.267mcg/ml respectively. The percentage assay of Vilazodone was 100.15%. The method was validated by determining its system suitability, precision, accuracy, and robustness parameters.. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate; this is useful for the routine determination of Vilazodone in bulk drug and in its pharmaceutical dosage form. Top Keywords Vilazodone, Calibration curve, RP-HPLC, Estimation, and Tablets. Top |