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Development and Validation of new RP-HPLC method for the estimation of Secnidazole in pure and pharmaceutical dosage forms Ajmeera Sunitha Rani* Chilkur Balaji College of Pharmacy, Moinabad, Hyderabad, R. R. Dist, India *Corresponding Author E-mail: sunitha.ajmera@gmail.com.
Online published on 26 March, 2018. Abstract A simple, precise, accurate RP-HPLC method was developed and validated for the estimation of Secnidazole in pure and pharmaceutical dosage form. An Agilent C8 column (250 mm x 4.6 mm), 5 μ particle size was used as stationary phase with mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 85: 15, V/V. The flow rate was maintained at 1 mL/min and effluents were monitored at 228 nm. The retention time was 7.995 min. The linearity of the method was observed in the concentration range of 80–120 μg/mL with correlation coefficient of 0.999. The percentage assay of Secnidazole was 99.60%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Secnidazole in pure and pharmaceutical dosage forms. Top Keywords Secnidazole, RP-HPLC, Phosphate buffer and Validation. Top | |
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