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Year : 2017, Volume : 10, Issue : 3
First page : ( 349) Last page : ( 353)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2017.00059.1

Evaluation of residues of chlorpyrifos and its metabolite in green apples

Rao Tentu Nageswara1, Parvatamma Botsa2, Apparao Karri1, Mastan Jakka1

1Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.

2Department of Organic Chemistry, Gayathri PG College, Vizianagaram, Andhra Pradesh, India.

Online published on 22 September, 2017.


A simple and inexpensive method was developed using solid-phase extraction, together with gas chromatographic method and high performance liquid chromatographic method for determination of chlorpyrifos and its metabolite O,O-diethyl triphosphate residues in green apples. The evaluated parameters include the extracts by silica gel packed column using methanol and water solvent mixture (90:10) and ethyl acetate solvents. The method was validated using green apple fruit samples spiked with chlorpyrifos and its metabolite (O,O-diethyl triphosphate) at different fortification levels (0.01 and 0.1 mg/kg). Average recoveries (using each concentration six replicates) of range 86–95%. The calibration solutions concentration in the range 0.01–5.0 mg/L and limit of detection (LOD) and limit of quantification (LOQ) were 0.003mg/kg and 0.01mg/kg respectively. Finally the green apple fruit residue samples were analyzed by GC and HPLC.



HPLC, GC, Silica gel, Chlorpyrifos, O,O-diethyl triphosphate and green apples.


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